RECIPES

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> CB 10X T4 DNA Ligase (+ATP) > Plasmid Preps > TE (Tris-EDTA Buffer) > Buffering Phenol > Enzyme Buffers > DNA gel loading buffer (5 X) > (TBE) Tris-Borate > TBE DNA gel running buffer (Tris borate EDTA) 10 X > Tris-Acetate (TAE) > Transcription Recipes

 

ENZYME BUFFERS

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Klenow Fragment: Storage buffer

100mM KH2PO4 (pH 7.0)	556uL of 0.9M
5mM DTT	25uL of 1M
50% glycerol	2.5mL

add some d H₂O, check pH, to 5 mL with n H₂O

10 X Mung Bean nuclease

0.3 M NaOAc pH 5.0

1 M NaCl

20 mM ZnCl2

 

10 X PHOSPHATASE (calf intestinal phosphatase)

0.2 M Tris 8.0

10 mM MgCl2

10 mM ZnCl2

use 0.1 U CIP to 1 to 20 pmoles DNA ends