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RECIPES > DNA

> CB 10X T4 DNA Ligase (+ATP)

> Plasmid Preps

> TE (Tris-EDTA Buffer)

> Buffering Phenol

> Enzyme Buffers

> DNA gel loading buffer (5 X)

> (TBE) Tris-Borate

> TBE DNA gel running buffer (Tris borate EDTA) 10 X

> Tris-Acetate (TAE)

> Transcription Recipes

 

TE (Tris-EDTA Buffer)

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  100mL 500mL
0.01M Tris 0.5ml 2.5mL (2M Stock)
0.001M EDTA 0.2mL 1mL (0.5M Stock)

make up to required volume with n H2O. pH = 8.0