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> CB 10X T4 DNA Ligase (+ATP) > Plasmid Preps > TE (Tris-EDTA Buffer) > Buffering Phenol > Enzyme Buffers > DNA gel loading buffer (5 X) > (TBE) Tris-Borate > TBE DNA gel running buffer (Tris borate EDTA) 10 X > Tris-Acetate (TAE) > Transcription Recipes

 

CB 10X T4 DNA Ligase (+ATP)

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0.66M Tris pH 7.6	0.5mL 2M Tris pH 7.6
0.1M MgCl2	0.15mL 1M MgCl2
0.05M DTT	0.075mL 1M DTT
0.01M ATP	0.15mL 0.1M ATP pH 7.5
	0.625mL H2O

Store in 200 uL aliquots at -20º C.