RECIPES

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> CB 10X T4 DNA Ligase (+ATP) > Plasmid Preps > TE (Tris-EDTA Buffer) > Buffering Phenol > Enzyme Buffers > DNA gel loading buffer (5 X) > (TBE) Tris-Borate > TBE DNA gel running buffer (Tris borate EDTA) 10 X > Tris-Acetate (TAE) > Transcription Recipes

 

(TBE) TRIS-BORATE

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1 X

89 mM Tris base

89 mM boric acid

2 mM EDTA

for 1 litre 5 X

54 gm Tris base

27.5 gm boric acid

3.72 gm EDTA

should come to pH 8.3