> Reticulocyte Lysate and Wheat Germ Extracts
> Immunoprecipitation of translation products
> History and evolution of cell-free protein synthesizing systems
> Import Into Mitochondria
> Wheat Germ Cell-Free Translation
> Protocol for wheat germ cell-free translation
> Wheat Germ Extract Buffers and reagents
> Sample WG Protocol
> Wheat Germ Extract Recipes
> Troubleshooting Wheat Germ Translation Reactions
Get a printable version of this protocol (requires Adobe Acrobat)
As with rabbit reticulocyte lysates (RRL), wheat germ (WG) extracts can be used to program the synthesis of a protein encoded in purified mRNA either native or cell-free transcribed. Read the general introduction to translation for more details.