> Translation Protocol
> Sample Protocol
> Troubleshooting
> Lysate Preparation of S-30
> Membrane-Free Ribosomes (mfrs)
> Ribosomal Pellet
> Dialysis Tubing Work-up
> Membrane-Free Translation System
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Divide into 3 1L flasks, 5-100ml flasks, and 5-100ml bottles, autoclave. Prior to inoculation, add 40ml of 25% filtered glucose per litre of media.
Autoclave, allow to cool to < 60C, then add 200ml of sterile, filtered 0.17M KH2PO4, 0.72M K2HPO4.