> Translation Protocol
> Sample Protocol
> Troubleshooting
> Lysate Preparation of S-30
> Membrane-Free Ribosomes (mfrs)
> Ribosomal Pellet
> Dialysis Tubing Work-up
> Membrane-Free Translation System
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This is incubated at 37°C for 45 min. for coupled T1 andT2
Note: Each batch of S-30 must be optimized for Mg (OAc)2 and K(glutamate)concentration.
