CELL FREE SYSTEMS	> TRANSLATION	> E coli Translation	> Translation Protocol
			> Sample Protocol
			> Troubleshooting
			> Lysate Preparation of S-30
			> Membrane-Free Ribosomes (mfrs)
			> Ribosomal Pellet
			> Dialysis Tubing Work-up
			> Membrane-Free Translation System
			> Buffers and Reagents

 

Membrane-Free Translation System

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As mentioned, the S-30 system contains endogenous E. coli membrane vesicles. In many cases, these may be undesirable, since it may be that membranes are to be added exogenously. To remove vesicles, a centrifugation at 135 000xg (59 000 rpm in TLA 100) is performed, and the supernatant retained as the "S135" fraction.