DNA	> DNA ISOLATION AND PLASMID PURIFICATION	> Phenol extraction and ethanol precipitation.
		> Large Scale Plasmid Prep for 100 ML Superbroth Cultures
		> Plasmid Miniscreen Protocol (Boil Prep)
		> Alkaline Lysis Miniprep
		> Alkaline Lysis Miniprep Modified 1
		> Alkaline Lysis Miniprep Modified 2
		> Amplification of plasmids with chloramphenicol
		> Single-Strand DNA Prep. Large Scale
		> Titre of Bacteriophage
		> Large Scale Bacteriophage Prep

Alkaline Lysis Miniprep

version reputed for its high yield

1.    Grow cells and pellet as before (30s in microfuge)
2.    Resuspend in 150 ul of cold SET, vortexing vigourously
3.    Add 350 ul of Solution II, invert until clear. Set on ice for 5 minutes
4.    Add 250 ul cold NaOAC. Hand Shake. Back on ice for 10 minutes
5.    Microfuge 5 minutes at room temperature or at 4^oC
6.    Remove supernatant into fresh tube
7.    Add 750 ul isopropanol, mix and microfuge at room temperature for 5 minutes
8.    Wash with 500 ul of 70% ethanol
9.    Resuspend in 20 ul TE+RNase A 

SET solution	for 100 ml
20% sucrose	20 g sucrose
15 mM Tris pH 8.0		750 ul 2M Tris
50 mM EDTA pH 8.0		10 ml 0.5M EDTA

Store at 4^oC.

RNase A solution

2 mg/ml, heated at 80^oC for 15 minutes, stored frozen

Solution II	for 100 ml
0.2 M NaOH	5 ml of a 4M NaOH stock
1% SDS	5 ml of a 20% SDS stock

NaOAC

3M sodium acetate, pH 5.2 with acetic acid, store at 4^oC