RECIPES

> POLYACRYLAMIDE GELS

> Laemmli Gels (SDS-PAGE) > Gel running/processing buffers > SDS-PAGE loading buffer

 

SDS-PAGE loading buffer

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WG/ab pptn

4% SDS

0.1 M Tris pH 8.9

2 mM EDTA

0.1% bromophenol blue

20% glycerol

0.25 M DTT (added fresh before use)

Retic

as above but with 10% SDS and 0.5 M DTT