Protocol for small scale induction to test expression
levels
This protocol is used to do a time course or other optimizations prior to scale
up to optimize overexpression.
For example a sample time course in minutes might be:
t= 0,30,60,90,120,...
post induction
Transform cells and pick a fresh colony into 5 mls LB amp
Grow cells to saturation overnight
Dilute cells 1:10 (200 microliters in 2 ml) with LB amp and grow further 180min (3 hrs) at 37ºC.
Induce with 1mM final IPTG (100mM stock 23.8 mg/ml)
Grow 2 hours more at 37ºC.
Boil prep (see small scale protocol )
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