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T4 Polymerase for Blunting 3' Extensions

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10X T4 buffer
500mM Tris pH 8
50 mM MgCl2
50mM DTT
500ug/mL BSA
For 50uL reactions:
CB10XT4 5uL
dNTP 5uL
DNA 5ug
T4 pol 0.2 units

Incubate 10 min room temp.

Add 2ul 0.5M EDTA

Heat to 75ºC for 10 min.

Ethanol precipitate.